High-Performance Liquid Chromatography (HPLC)


High-performance liquid chromatography (HPLC) is a chromatographic method which is usually used to separate, identify and quantify the components in a mixture. The substance to be examined is to be solved in a solvent or solvent mixture (mobile phase) and moves through a column filled with a solid adsorbent material (stationary phase). A suitable detector generates a signal proportional to the amount of sample component emerging from the column, hence allowing for quantitative analysis of the sample components. In contrast to GPC-measurements (highest priority should be given to avoid

interactions between the dissolved species, the solvent and the column material) in HPLC-measurements the components of the sample move through the column at different velocities, which are a function of specific physical interactions with the adsorbent. The components in a sample interacts slightly differently with the stationary phase, causing different flow rates for the different components and leading to the separation of the components. That effect can be used to separate polymers with the same molecular weight but differences in their chemical composition (copolymers, polymer blends). HPLC is very often used to estimate the content and kind of low molecular substances in polymers (inhibitors, oils, plasticizers, accelerators).

(in cooperation with the University of Applied Sciences Merseburg)


Sample Preparation

  • required sample amount: 10 – 20 mg
  • the appropriate amount of sample are weighed into sample vials and treated with a suitable solvent (concentration: approximately 1 mg/ml or less)
  • sample solution will be filtered (0.2 µm syringe filter) and injected into the HPLC-system

Testing Conditions

  • measuring temperature: 25 – 60 °C (depends on the used solvent)
  • only dissolved polymer molecules can be detected

Components of our HPLC-system

  • SECcurity quaternary gradient pump 
  • SECcurity automatic sample changer
  • SECcurity HPLC column thermostat TCC6000
  • different HPLC columns
  • SECcurity multi wavelength UV-VIS detector 
  • SECcurity ELSD detector

Contact

Dr. Zhanna Evgrafova
Telefon: +49 (0)3461 46-2103
zhanna.evgrafova@psm-merseburg.de

Prof. Dr. Valentin Cepus
Telefon: +49 (0)3461 30889-51
valentin.cepus@psm-merseburg.de

Quickinfo

Results

  • Separation of dissolved components
  • Separation of polymers with the same molar mass but different chemical composition
  • Separation of low molecular substances in polymers