Gel Permeation Chromatography (GPC)

Bild der GPC-AnlageGel permeation chromatography (GPC) or size exclusion chromatography (SEC) is a liquid chromatographic technique in which macromolecules (oligomers and polymers) are separated based on their hydrodynamic volume. This happens with the help of a highly porous polymeric column material. SEC is the method of choice for the determination of the molar mass and molar mass distribution of natural and synthetic macromolecules. The combination of different detectors provides important information concerning the chemical composition and the molecular architecture of polymers.

A GPC-system normally consists of an isocratic HPLC pump, an injection system (automatically or manually), a high resolution column or a combination of columns and at least one detector. The combination of concentration detectors (RI or UV), with a viscosity and a MALS-detector allows a comprehensive characterization of unknown polymer samples (absolute molecular weight, molecular-mass distribution, intrinsic viscosity, radius of gyration). The information allows conclusions on the structure, composition, degree of substitution and the branching of polymers as a function of molar mass.

(in cooperation with the University of Applied Sciences Merseburg)

Sample Preparation

  • the appropriate amount of sample are weighed into sample vials and treated with THF
  • after 24h the sample solution will be filtered (0.2 µm syringe filter) and injected into the GPC-system

Testing Conditions

  • measuring temperature: 25 – 40 °C
  • only dissolved polymer molecules can be detected

Components of our GPC-system

  • SECcurity quaternary gradient pump 
  • SECcurity automatic sample changer
  • SECcurity GPC column thermostat TCC6000
  • PSS SDV combination of columns  (separation range: 100 - 3 000 000 Da)
  • SECcurity multi wavelength UV-VIS detector 
  • SECcurity RI detector
  • SECcurity MALS SLD7100
  • SECcurity GPC viscosity detector DVD1260


Dr. Zhanna Evgrafova
Telefon: +49 (0)3461 46-2103



  • Molar mass
  • Molar mass distribution
  • Chemical composition
  • Molecular architecture
  • Intrinsic viscosity